{
  "id": "lib-process:bioinformatics--crispr-screen-analysis",
  "_kind": "LibraryProcess",
  "_file": "generated-library/processes.yaml",
  "_cluster": "generated-library",
  "attributes": {
    "displayName": "crispr-screen-analysis",
    "description": "CRISPR Screen Analysis - Analyzing pooled CRISPR knockout/activation/inhibition screens\nfor gene essentiality, synthetic lethality, and functional genomics applications.",
    "libraryPath": "library/specializations/domains/science/bioinformatics/crispr-screen-analysis.js",
    "specialization": "bioinformatics",
    "references": [
      "- MAGeCK: https://sourceforge.net/p/mageck/wiki/Home/\n- BAGEL2: https://github.com/hart-lab/bagel\n- CRISPRcleanR: https://github.com/francescojm/CRISPRcleanR\n- CRISPR-ScreenProcessing: https://github.com/mhegde/CRISPR-Analysis\n- DepMap: https://depmap.org/"
    ],
    "example": "const result = await orchestrate('specializations/domains/science/bioinformatics/crispr-screen-analysis', {\n  projectName: 'Drug Resistance Screen',\n  screenType: 'knockout',\n  libraryInfo: { name: 'Brunello', guides: 76441 },\n  sequencingFiles: ['/data/plasmid.fastq', '/data/t0.fastq', '/data/treatment.fastq']\n});",
    "usesAgents": [
      "general-purpose"
    ]
  },
  "outgoingEdges": [
    {
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      "to": "skill-area:data-analysis",
      "kind": "lib_requires_skill_area",
      "attributes": {
        "weight": 1
      }
    },
    {
      "from": "lib-process:bioinformatics--crispr-screen-analysis",
      "to": "skill-area:statistical-analysis",
      "kind": "lib_requires_skill_area",
      "attributes": {
        "weight": 0.7
      }
    },
    {
      "from": "lib-process:bioinformatics--crispr-screen-analysis",
      "to": "skill-area:python-data-pipelines",
      "kind": "lib_requires_skill_area",
      "attributes": {
        "weight": 0.5
      }
    },
    {
      "from": "lib-process:bioinformatics--crispr-screen-analysis",
      "to": "domain:bioinformatics",
      "kind": "lib_applies_to_domain",
      "attributes": {
        "weight": 1
      }
    },
    {
      "from": "lib-process:bioinformatics--crispr-screen-analysis",
      "to": "role:research-engineer",
      "kind": "lib_involves_role",
      "attributes": {
        "weight": 1
      }
    },
    {
      "from": "lib-process:bioinformatics--crispr-screen-analysis",
      "to": "role:biomedical-engineer",
      "kind": "lib_involves_role",
      "attributes": {
        "weight": 0.7
      }
    },
    {
      "from": "lib-process:bioinformatics--crispr-screen-analysis",
      "to": "workflow:experiment-design",
      "kind": "lib_implements_workflow",
      "attributes": {
        "weight": 1
      }
    },
    {
      "from": "lib-process:bioinformatics--crispr-screen-analysis",
      "to": "specialization:biomedical-informatics",
      "kind": "lib_belongs_to_specialization",
      "attributes": {
        "weight": 1
      }
    },
    {
      "from": "lib-process:bioinformatics--crispr-screen-analysis",
      "to": "specialization:bioinformatics",
      "kind": "lib_belongs_to_specialization",
      "attributes": {
        "weight": 0.9
      }
    }
  ],
  "incomingEdges": []
}